Secretory microRNA profiles of third- and fourth-stage Dirofilaria immitis larvae with different macrocyclic lactone-susceptibility: in search for biomarkers for early-detection of infection

We cultured D. immitis L3 and L4 of two laboratorial strains with different susceptibility status to macrocyclic lactone drugs in vitro. Excretory/secretory microRNAs were sequenced and analyzed. Overall design: D. immitis L3 and L4 of two different strains were allowed to excrete/secrete miRNAs in vitro, which were profiled by miRNA sequencing and compared.

本研究体外培养了两株对大环内酯类药物（macrocyclic lactone drugs）易感性存在差异的实验室犬恶丝虫（Dirofilaria immitis，缩写D. immitis）菌株的第三期幼虫（L3）与第四期幼虫（L4），并对其排泄/分泌型微小RNA（microRNA, miRNA）开展了测序与分析。整体实验设计：将两株不同菌株的犬恶丝虫L3与L4置于体外环境中培养以收集其排泄/分泌的miRNA，通过miRNA测序构建miRNA表达谱并进行组间比较。