CRISPR deletion of ELAVL1 (HuR) in MIA PaCa-2 in vitro cells and in vivo tumors

To investigate the impact of the mRNA stabilty factor HuR on the pancreatic cancer transcriptome in MIA PaCa-2 in vitro cell line and in vivo tumors at multiple time points of tumor growth CRISPR-mediated deletion of ELAVL1 (HuR) in MIA PaCa-2 PDAC cells. HuR wildtype (WT) or HuR knockout (KO) cells in culture (in vitro) were collected at 70% confuency for RNA extraction. HuR WT and HuR KO orthotopic tumors (in vivo) were harvested 2, 3, and 4 weeks post-injection for RNA extraction.

为探究mRNA稳定性因子HuR对MIA PaCa-2体外细胞系及多个肿瘤生长时间点下体内肿瘤的胰腺癌细胞转录组的影响，本研究通过CRISPR介导技术在MIA PaCa-2胰腺导管腺癌（PDAC）细胞中敲除ELAVL1（HuR）。收集处于70%汇合度的体外培养的HuR野生型（WT）与HuR敲除型（KO）细胞用于RNA提取；于细胞接种后2、3、4周时，收集HuR野生型与敲除型原位肿瘤（体内）样本以提取RNA。