The Evolving Immune Microenvironment of B-cell Acute Lymphoblastic Leukemia [murine dataset]

Single-cell RNA-seq analysis of murine bone marrow and peripheral blood mononuclear cells (PBMCs) immune populations isolated from B-ALL recipient animals and two healthy littermate controls. scRNA-Seq: For healthy recipients, processed bone marrow or peripheral blood cells were separated into GFP-CD45+Ly-6G- non-granulocytic cells and GFP-CD45+Ly-6G- non-granulocytic cells, then mixed at a ratio of 8 parts GFP-CD45+Ly-6G- non-granulocytic cells and 1 part GFP-CD45+Ly-6G+ granulocytic cells. For B-ALL recipients, cells were sorted and mixed to give 1 part GFP+CD19+ B-ALL blasts, 8 parts GFP-CD45+Ly-6G- non-granulocytic cells, and 1 part GFP-CD45+Ly-6G+ granulocytic cells.

本数据集为分离自B细胞急性淋巴细胞白血病（B-cell acute lymphoblastic leukemia, B-ALL）受体动物以及2只健康同窝对照小鼠的小鼠骨髓与外周血单个核细胞（peripheral blood mononuclear cells, PBMCs）免疫细胞群的单细胞RNA测序（single-cell RNA sequencing, scRNA-seq）分析数据。实验处理细节如下：对于健康受体动物，将经预处理的骨髓或外周血细胞分为绿色荧光蛋白（green fluorescent protein, GFP）阴性CD45阳性Ly-6G阴性非粒细胞群与GFP-CD45+Ly-6G+粒细胞群，随后以8份GFP-CD45+Ly-6G-非粒细胞与1份GFP-CD45+Ly-6G+粒细胞的比例进行混合；对于B-ALL受体动物，则将分选获得的细胞按1份GFP+CD19+ B-ALL母细胞、8份GFP-CD45+Ly-6G-非粒细胞及1份GFP-CD45+Ly-6G+粒细胞的比例进行混合。