Expression data from NRK-52E cells treated with chlorothalonil for 6h, 24h and 72h.

Chlorothalonil (2,4,5,6-tetrachloroisophthalonitrile) is a widely used broad spectrum fungicide. Despite its common use, chlorothalonil is classified by IARC as Group 2B, a possible human carcinogen, with formation of renal tubular adenomas and carcinomas in rats of both sexes and in male mice. In this study we have examined the effect of sub-cytotoxic exposure to chlorothalonil at 6h, 24h and 72h on the whole-genome expression profile in a rat proximal renal tubule cell line (NRK-52E). We used microarrays to detail the mechanism of toxicity and possibly carcinogenicity. NRK-52E cells were cultured to confluence on 6-well plates. Cells were then exposed to chlorothalonil dissolved in DMSO (0.1%) at the IC10 concentration at 72h (1.1 μM CHL) or DMSO only as control. After 6h, 24h and 72h the medium was removed and RNA was extracted from the cells. Three studies were conducted at each time point.

百菌清（Chlorothalonil，化学名2,4,5,6-四氯间苯二腈）是一种应用广泛的广谱杀菌剂。尽管其应用较为普遍，百菌清仍被国际癌症研究机构（IARC）划分为2B类致癌物，即潜在人类致癌物，且可在雌雄大鼠与雄性小鼠体内诱发肾小管腺瘤与肾小管癌。本研究以大鼠近端肾小管细胞系（NRK-52E）为模型，探究了分别经亚细胞毒性剂量百菌清暴露6h、24h、72h后，细胞全基因组表达谱的变化情况。本研究采用基因微阵列（microarrays）技术，解析百菌清的毒性及潜在致癌性作用机制。实验过程中，NRK-52E细胞在6孔板中培养至汇合状态，随后分别使用溶于0.1%二甲基亚砜（DMSO）的72h时10%抑制浓度（IC10）的百菌清（1.1 μM CHL）溶液，以及仅含0.1% DMSO的培养基进行处理作为空白对照。分别在暴露6h、24h、72h后移除培养基，提取细胞内总RNA。每个时间点均设置3次重复实验。