Glycosylation-related gene expression in the mucus-secreting gastrointestinal cell line HT29-MTX-E12 in response to infection by Helicobacter pylori. Homo sapiens

The HT29 derivative cell line HT29-MTX-E12 (E12) produces an adherent mucus layer predominantly of the gastric MUC5AC mucin when grown on transwells. This mucus layer supports Helicobacter pylori survival in culture. E12 cells were infected with H. pylori and the transcriptome of infected and uninfected E12 were compared. Also included for comparison was the HT29 parent cell line grown on transwells. The study focused on glycosylation related genes as these regulate the structures that have a major role to play in pathogen-host cell interaction. Overall design: E12 cells were infected with H. pylori and the transcriptome of infected and uninfected E12 were compared.

HT29衍生细胞系HT29-MTX-E12（以下简称E12）在Transwell小室（Transwell）中培养时，可形成以胃源性MUC5AC黏蛋白为主要组分的黏附性黏液层。该黏液层可支持幽门螺杆菌（Helicobacter pylori）在体外培养环境中存活。研究人员将E12细胞感染幽门螺杆菌，并对比感染与未感染状态下E12细胞的转录组。本次研究同时纳入在Transwell小室中培养的HT29亲本细胞系作为对照。本研究聚焦于糖基化相关基因，因为这类基因可调控在病原菌-宿主细胞互作中发挥关键作用的分子结构。实验整体设计：将E12细胞感染幽门螺杆菌，并对比感染与未感染状态下E12细胞的转录组。