Connecting cellular water behaviour to drug action

Considering that the dynamical window covered by IRIS (or OSIRIS) is within the observed diffusion coefficient range for intracellular water, this proposal aims to achieve a much better understanding of the behaviour of cellular water before and after being treated by the monoclonal antibody tocilizumab (TCZ); either free of, or encapsulated in, a newly developed biopolymeric nanoparticle. It is expected that cells treated with free or encapsulated TCZ, as opposed to cells treated only with the NPs, will present a different flexibility when compared to untreated cells (control).

鉴于IRIS（或OSIRIS）覆盖的动态窗口处于细胞内水观测到的扩散系数范围内，本提案旨在更深入地理解细胞内水在经单克隆抗体托珠单抗（TCZ）处理前后的行为——TCZ可为游离形式，或包裹于新开发的生物聚合物纳米颗粒中。预期经游离或包裹形式TCZ处理的细胞，与仅经纳米颗粒（NPs）处理的细胞相比，其柔性将与未处理细胞（对照组，control）呈现差异。