The study of SWI/SNF subunits and other transcription factors deposition in asynchronous and mitotic mESCs and cells released from mitosis II. The study of SWI/SNF subunits and other transcription factors deposition in asynchronous and mitotic mESCs and cells released from mitosis II

We conduct ATAC-seq in SMARCE1 MD/MD(R42A) after mitotic release 90 min in either DMSO treated or BRG1 inhibitor BRM014 treated conditions Overall design: Examination of chromatin accessibility in mitotically degraded SMARCE1 and control on either DMSO or BRG1 inhibitor BRM014 treated ES cells.

我们在经二甲基亚砜（DMSO）处理或BRG1抑制剂BRM014处理的两种条件下，对有丝分裂释放90分钟后的SMARCE1 MD/MD(R42A)样本开展转座酶可及性测序（ATAC-seq）。整体实验设计：检测经二甲基亚砜（DMSO）或BRG1抑制剂BRM014处理的胚胎干细胞（ES细胞）中，经有丝分裂降解的SMARCE1与对照样本的染色质可及性。