Total RNA-seq from HCT116, MCF7 and SJSA cell lines treated with DMSO and Nutlin

To determine effects of p53 activation on steady-state mRNA levels, we performed RNA-seq analysis of colorectal carcinoma cell line HCT116 (p53+/+ and p53 -/-), breast carcinoma line MCF7, and osteosarcoma line SJSA treated with MDM2 inhibitor Nutlin. Overall design: Total RNA was sequenced from HCT116 (p53+/+ and p53 -/-), MCF7 and SJSA cells treated with DMSO or 10 microM Nutlin for 12 hours.

为探究p53激活对细胞稳态mRNA水平的影响，我们对经MDM2抑制剂Nutlin处理的结直肠癌细胞系HCT116（p53+/+与p53-/-）、乳腺癌细胞系MCF7以及骨肉瘤细胞系SJSA开展了RNA测序（RNA-seq）分析。
实验整体设计：提取经二甲基亚砜（DMSO）或10 μM Nutlin处理12小时的HCT116（p53+/+与p53-/-）、MCF7及SJSA细胞的总RNA，随后进行测序。