Whole genome transcriptional profile of human tissues and cells

We performed tiling array experiments to examine whole genome expression in human tissues. We investigated tissue specificity and association between evolutionary sequence conservation and transcription. A total of 22 tissues (bone marrow, cerebellum, colon, cortex, fetal brain, heart, kidney, liver, lung, pancreas, prostate, salivary gland, skeletal muscle, small intestine, spinal cord, spleen, stomach, testes, thymus, thyroid, trachea and uterus) and 2 cell lines (HeLa and SH-SY5Y) were examined in 36783546 regions of the Human genome in this study. Keywords: whole genome transcription, tiling array, non-coding transcripts, tissue specificity Total RNA isolated from Human tissues and cell cultures were labeled and hybridized to the GeneChip Human Tiling 1.0R Array set of 14 chips (24 samples x 14 = 336 chips total) according to the manufacturer's protocol.

本研究通过tiling 阵列（tiling array）实验，检测人类组织中的全基因组表达水平，同时探究了转录的组织特异性，以及进化序列保守性与转录过程之间的关联。
本研究共检测人类基因组中36783546个区域，涉及22种组织（骨髓、小脑、结肠、大脑皮层、胎儿脑组织、心脏、肾脏、肝脏、肺、胰腺、前列腺、唾液腺、骨骼肌、小肠、脊髓、脾脏、胃、睾丸、胸腺、甲状腺、气管与子宫）以及2种细胞系（HeLa与SH-SY5Y）。
关键词：全基因组转录、tiling 阵列、非编码转录本、组织特异性
本研究按照制造商提供的实验流程，对从人类组织与细胞培养物中分离得到的总RNA进行标记，随后与包含14张芯片的GeneChip Human Tiling 1.0R阵列组（24个样本×14张芯片，总计336张芯片）进行杂交。