Transcriptome analyses of GPR39-overexpressed and GPR39-activated HEK293 cells

To get insight of molecular mechanism of GPR39, we investigated transcriptomic response in GPR39 overexpressed HEK293 cells after TC-G-1008 stimulation We identified 814 differentially expressed genes (DEGs) fold change cutoff = 1.5 in the GPR39 overexpressed HEK293 and GPR39 overexpressed HEK293 cells with TC-G-1008 treatment, those including 364 up-regulated and 450 down-regulated genes These findings suggest that MAPK/Erk, PI3K/Akt and Glycerolipid metabolism signaling pathways would be putative signaling pathways dominantly altered by GPR39 activation. HEK293 cell line was treated in DMEM with 10% FBS (Gibco) with Cumate 0.03mg/mL or TC-G-1008 (1uM)

为深入解析GPR39的分子作用机制，本研究针对经TC-G-1008刺激后的GPR39过表达HEK293细胞开展转录组应答分析。本研究在GPR39过表达HEK293细胞与经TC-G-1008处理的GPR39过表达HEK293细胞中，共鉴定出814个差异表达基因（differentially expressed genes, DEGs），差异倍数阈值设定为1.5，其中上调基因364个，下调基因450个。上述研究结果表明，MAPK/Erk、PI3K/Akt及甘油脂代谢信号通路或为GPR39激活后发生显著改变的核心推定信号通路。本研究中，HEK293细胞系培养于添加10% Gibco胎牛血清的DMEM培养基内，并分别以0.03mg/mL的Cumate或1μM的TC-G-1008进行处理。