Constituents of Prokaryotic Organism

Research was to study exoenzyme a extracellular enzyme secretions by a cell and functions outside of organism. The research question was this for prokaryotic and eukaryotic organisms important for many biological processes. The method involved studies of cytoplasm within a cell surround by membrane. This consisted of examination of the material a gelatinous substance, organelles, sub-structures and inclusions. The results showed most prokaryotic had sizes from 0.5 to 2.0μm in diameter. This had three regions the membrane and some bacteria. The cellular concentrations had levels of 6 to 8 degrees of magnitude lower than those of high abundance of proteomes. The gene micro-arrays had interactions with oligo cells. It concluded phase extraction was needed in sets and preconcentration. The surrounds of glycan structures synthesis had presence of glycan in biological samples of immunologically based measurements of cells.

本研究旨在研究外酶（exoenzyme）——由细胞分泌、在生物体外部发挥功能的胞外酶。本研究的核心议题为：原核生物与真核生物中的外酶是否在诸多重要生物学过程中发挥关键作用。本研究采用的实验方法包括对细胞膜包被的细胞内细胞质进行分析，具体涵盖对细胞质内胶状基质、细胞器、亚结构与内含物的观测与分析。研究结果显示，多数原核生物的直径介于0.5至2.0微米之间。此类原核生物的细胞结构包含三个区域，即细胞膜及相关细菌组分。胞外酶的细胞浓度相较于高丰度蛋白质组的浓度低6至8个数量级。基因微阵列（gene micro-arrays）可与寡核苷酸细胞（oligo cells）发生特异性相互作用。本研究得出结论：需采用分批相萃取与预浓缩手段。在糖链（glycan）结构合成的周边微环境中，采用免疫学方法检测的细胞生物样本内可检出糖链的存在。