Global transcriptomic changes following elevated carbon dioxide (5%) in the wild-type (WT) and mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) Nannochloropsis oceanica IMET1: Illumina sequencing. Global transcriptomic changes following elevated carbon dioxide (5%) in the wild-type (WT) and mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) Nannochloropsis oceanica IMET1: Illumina sequencing

In Nannochloropsis oceanica IMET1, transcript knockdown of a cytosolic carbonic anhydrase (CA2; g2018) specifically inhibited by HC resulted in ~45%, ~30% and ~40% elevation of photosynthetic oxygen evolution rate, growth rate and biomass accumulation rate under high CO2 (5% ), respectively. This CA2-knockdown mutant is demonated as M2. To probe mechanistic links underlying the mutant (M2; RNAi-knockdown line of carbonic anhydrase (CA2)) phenotypes, temporal transcriptomic profiles are compared between RNAi-knockdown line of carbonic anhydrase (CA2) and WT, at 12 h and 24h under high CO2 (5%). Overall design: Nannochloropsis oceanica IMET1 wild-type and M2 (RNAi-knockdown line of carbonic anhydrase (CA2)) cells were grown in liquid cultures under continuous light (approximately 80±5 µmol photons m-2 s-1) at 25℃ and aerated by bubbling with a mixture of 5% CO2 in air. Mid-logarithmic phase algal cells were collected and washed three times with axenic seawater. Equal numbers of cells were re-inoculated in fresh medium and cultured under 5% CO2. Cell aliquots of WT and M2 were collected for RNA isolation after being transferred to the fresh medium conditions for 12h and 24h. Three biological replicates were randomly selected to prepare mRNA-Seq library. In total, 12 samples collected at two time points (12h and 24h) were used for mRNA-Seq library preparation and then submitted to Illumina HiSeq 2000 for sequencing. Please note that [1] each FPKM.txt processed data file contains 3 data columns (one for each replicate) and is linked to the corresponding 'replicate1' sample records, [2] the M2_WT_*FPKM.txt files contain the merged data of each sample group for the indicated time point and are linked as Series supplementary files.

在海洋微拟球藻IMET1（Nannochloropsis oceanica IMET1）中，通过HC特异性抑制胞质碳酸酐酶（cytosolic carbonic anhydrase, CA2；基因编号g2018）的转录敲低（transcript knockdown），可在5%高浓度二氧化碳（high CO2）条件下分别使光合放氧速率、生长速率及生物量积累速率提升约45%、30%和40%。该CA2转录敲低突变体命名为M2。为探究该CA2敲低突变体（M2；CA2的RNA干扰敲低株（RNAi-knockdown line））表型背后的分子机制关联，本研究对5%高浓度二氧化碳条件下培养12 h与24 h的CA2 RNA干扰敲低株与野生型（wild-type, WT）菌株进行了时间维度转录组谱（temporal transcriptomic profiles）比较分析。实验整体设计如下：将海洋微拟球藻IMET1野生型菌株与M2置于液体培养基中培养，培养条件为25℃、持续光照（约80±5 μmol光子·m⁻²·s⁻¹），并通入体积分数为5%二氧化碳的空气混合气进行曝气培养。收集对数中期的藻细胞，用无菌海水（axenic seawater）洗涤三次后，以等量细胞接种至新鲜培养基中，继续在5%二氧化碳条件下培养。分别于转接新鲜培养基后的12 h与24 h，收集野生型与M2菌株的细胞样品用于RNA提取。随机选取3个生物学重复样品制备mRNA测序文库（mRNA-Seq library）。本研究共收集2个时间点（12 h与24 h）下的12份样品，用于mRNA-Seq文库构建，随后送至Illumina HiSeq 2000测序平台（Illumina HiSeq 2000）进行测序。请注意：[1] 每份FPKM.txt格式的处理后数据文件包含3列数据（对应3个生物学重复），并与对应的'replicate1'样品记录相关联；[2] 命名格式为M2_WT_*FPKM.txt的文件包含指定时间点下各样品组的合并数据，作为系列补充文件进行关联提供。