Transcriptome-wide m6A Methylation Profiling of dHL-60 human neutrophils by m6A-seq

By performing m6A-seq analysis on dHL-60 human neutrophils infected with Escherichia coli, we want to identify the m6A methylation pattern in neutrophils after bacteria challenge. m6A-seq analysis revealed that the consensus m6A motifs were most significantly enriched within the m6A peaks with typical m6A peak distribution features, and m6A methylation sites were located primarily in the protein coding sequence region and 3′ untranslated region of mRNA transcripts, in neutrophils with bacterial infection. Identifying the m6A methylation pattern in dHL-60 human neutrophils infected with Escherichia coli. Two biological replicates.

本研究通过对感染大肠杆菌（Escherichia coli）的dHL-60人中性粒细胞开展m6A测序（m6A-seq）分析，旨在明确细菌刺激后中性粒细胞内的m6A甲基化修饰模式。m6A测序分析结果显示，在具备典型m6A峰分布特征的m6A峰区域内，共识性m6A基序的富集程度最为显著；在受细菌感染的中性粒细胞中，m6A甲基化位点主要分布于mRNA转录本的蛋白质编码序列区域与3′非翻译区（3′ untranslated region）。本数据集的研究对象为感染大肠杆菌的dHL-60人中性粒细胞，共设置2组生物学重复。