The transcriptional regulation analysis of ETS2 in mouse BMM cells (Cut&Tag)

The transcription factor ETS2 was identified as a hub gene that promotes osteoclast differentiation during the progression of osteoarthritis (OA). Virtual perturbation and in vitro perturbation experiments demonstrated that knockdown of ETS2 can inhibit osteoclast differentiation. Transcriptional regulatory network analysis and combined CUT&Tag with ATAC-seq analysis results indicate that ETS2 promotes osteoclast differentiation by targeting and enhancing the expression of CEBPB. Overall design: Mouse BMM cells were induced for two days with medium containing 50 ng/ml RANKL and 50 ng/ml M-CSF. The Cut&Tag experiment was then performed using ETS2 antibodies to explore the transcriptional regulation of ETS2.

转录因子ETS2（transcription factor ETS2）被鉴定为骨关节炎（osteoarthritis, OA）进展过程中促进破骨细胞分化的枢纽基因。虚拟扰动与体外扰动实验证实，敲低ETS2可抑制破骨细胞分化。转录调控网络分析及联合CUT&Tag与ATAC-seq的分析结果表明，ETS2通过靶向并增强CEBPB的表达来促进破骨细胞分化。实验设计概述：将小鼠BMM细胞用含50 ng/ml RANKL与50 ng/ml M-CSF的培养基诱导培养两天，随后使用ETS2抗体开展CUT&Tag实验，以探究ETS2的转录调控作用。