Epithelial endoplasmic reticulum stress orchestrates a protective IgA response I. Epithelial endoplasmic reticulum stress orchestrates a protective IgA response I

Immunoglobulin A (IgA) is the major secretory immunoglobulin isotype at mucosal surfaces where it regulates microbial commensalism and excludes luminal factors from contacting intestinal epithelial cells (IEC). IEC endoplasmic reticulum (ER) stress induces a polyreactive IgA response which protects from small intestinal inflammation. IEC ER stress causes expansion and activation of peritoneal B1b cells independent of microbiota and T cells that culminates in increased lamina propria and luminal IgA. Xbp1dIEC mice exhibit IEC ER stress by conditional deletion of X-box-binding protein 1 (XBP1). Here we examine differentially expressed genes in peritoneal B1b cells of germ-free Xbp1dIEC mice compared to littermate (WT) controls. Overall design: Peritoneal B1b cell mRNA profiles of 10-week-old germ-free Xbp1dIEC or WT mice were generated by high throughput sequencing

免疫球蛋白A（Immunoglobulin A, IgA）是黏膜表面的主要分泌型免疫球蛋白同种型，可调控微生物群共生，并阻止肠腔因子接触肠上皮细胞（intestinal epithelial cells, IEC）。肠上皮细胞内质网（endoplasmic reticulum, ER）应激可诱导多反应性IgA应答，从而抵御小肠炎症。肠上皮细胞内质网应激会在不依赖微生物群与T细胞的前提下，促使腹膜B1b细胞扩增并活化，最终导致固有层与肠腔中的IgA水平升高。Xbp1dIEC小鼠通过条件性敲除X盒结合蛋白1（X-box-binding protein 1, XBP1），可诱导肠上皮细胞内质网应激。本研究对无菌Xbp1dIEC小鼠与其同窝野生型（WT）对照小鼠的腹膜B1b细胞进行差异表达基因分析。实验整体设计：采用高通量测序（high throughput sequencing）技术，获取10周龄无菌Xbp1dIEC或野生型（WT）小鼠的腹膜B1b细胞mRNA表达谱。