Schizosaccharomyces cryophilus OY26 WGS sequencing. Schizosaccharomyces cryophilus OY26

Before the official species description of Schizosaccharomyces cryophilus OY26 this strain was known as Schizosaccharomyces sp. strain OY26. The sequenced stain is a type strain and has been deposited at NRRL, Agricultural Research Service Culture Collection as NRRL Y-48691, at the Centraal Bureau voor Schimmelcultures as CBS 11777 and at NBRC, NITE Biological Resource Center as NBRC 106824. This strain was isolated as a contaminant of the Schizosaccharomyces octosporus strain CBS 7191. Genomic DNA from the S. cryophilus strain was submitted to 454 Life Sciences for whole-genome sequencing. A titanium fragment library was generated from 5 mg genomic DNA and sequenced on a single 454 run using titanium chemistry. A 3K long-tag paired-end (LTPE) library was generated in parallel from the same amount of genomic DNA and sequenced on half of an FLX run. Approximately 1.3 million high-quality reads of 400 bp were generated from the titanium run and 700 000 reads were generated from the LTPE FLX run. Quality-filtered sequences from the wholegenome shotgun fragment library and LTPE library were assembled using the 454 Newbler Assembler.

在嗜冷裂殖酵母（Schizosaccharomyces cryophilus）OY26正式发表物种描述之前，该菌株曾被称为裂殖酵母属（Schizosaccharomyces）未定种菌株OY26。该测序菌株为模式菌株，已分别保藏于NRRL（美国农业研究服务菌种保藏中心，Agricultural Research Service Culture Collection），保藏号为NRRL Y-48691；荷兰真菌保藏中心（Centraal Bureau voor Schimmelcultures），保藏号为CBS 11777；以及NBRC（日本NITE生物资源中心，NITE Biological Resource Center），保藏号为NBRC 106824。该菌株是作为八孢裂殖酵母（Schizosaccharomyces octosporus）菌株CBS 7191的污染物被分离得到的。本研究将嗜冷裂殖酵母菌株的基因组DNA提交至454 Life Sciences公司开展全基因组测序：以5 mg基因组DNA为材料构建钛试剂片段文库，采用钛试剂化学法通过单次454测序运行完成测序；同时以相同质量的基因组DNA并行构建3K长标签配对末端（LTPE）文库，并通过半次FLX测序运行完成测序。钛试剂测序运行共产生约130万条长度为400 bp的高质量读段（reads），LTPE FLX测序运行则产生70万条读段。最终通过454 Newbler组装软件对全基因组鸟枪法片段文库与LTPE文库的质量过滤序列进行拼接组装。