AEBP1-GLI1 pathway attenuates the FACT complex dependency of bladder cancer cell survival.

AEBP1 expression predicts a poor prognosis of bladder cancer patients, while its role has not been fully understood. We here show that the expression of AEBP1 was correlated with GLI transcriptional factors, leading to limit the SSRP1/SUPT16H (FACT) complex-dependency of bladder cancer cells. AEBP1-highly expressing human bladder cancer cell line 5637 was used for the RNA-seq experiment: cells were transfected with either AEBP1-specific siRNA siAEBP1-1 or its control siControl. 72 hours after transfection, cells were harvested for RNA exraction. The gene expression profile was then analyzed using data obtained from the RNA-seq of triplicate samples.

AEBP1的表达可预测膀胱癌患者的不良预后，但其具体作用尚未完全阐明。本研究发现，AEBP1的表达与GLI转录因子相关，进而可限制膀胱癌细胞对SSRP1/SUPT16H（FACT）复合物的依赖。本研究选用高表达AEBP1的人膀胱癌细胞系5637开展RNA测序（RNA-seq）实验：将细胞分别转染AEBP1特异性小干扰RNA（siRNA）siAEBP1-1及其阴性对照siControl。转染72小时后收集细胞进行RNA提取，随后基于三份重复样本的RNA测序数据分析基因表达谱。