Murine ex vivo cultured alveolar macrophages provide a novel tool to study tissue-resident macrophage behavior and function

MexAMs are functionally similar to primary AMs, so we compared their transcriptional profile to different macrophage populations. These included BMDMs, as the macrophage type predominantly used for in vitro studies, arising from myeloid bone marrow progenitor cells, as well as peritoneal macrophages (PMs), a mature TRM population exposed to a different tissue microenvironment. mRNA profiles of listed cell types (BALF, PLF, BMDM, mexAM)

MexAMs在功能上与原发性肺泡巨噬细胞（primary AMs）高度相似，因此我们将其转录组谱与多种巨噬细胞群开展了对比分析。本次比对涵盖的细胞类型包括：骨髓来源巨噬细胞（bone marrow-derived macrophages, BMDMs）——这是体外研究中应用最为广泛的巨噬细胞亚群，由骨髓髓系祖细胞分化产生；以及腹腔巨噬细胞（peritoneal macrophages, PMs）——一类暴露于不同组织微环境的成熟组织驻留巨噬细胞（tissue-resident macrophages, TRMs）。本数据集包含所列细胞群（支气管肺泡灌洗液巨噬细胞（bronchoalveolar lavage fluid macrophages, BALF）、肺灌洗液巨噬细胞（pulmonary lavage fluid macrophages, PLF）、BMDM、mexAM）的mRNA转录组谱。