Heritable epimutations induced during clonal propagation in Arabidopsis.

Clonally propagated plants are genetically invariant, but often display somaclonal variation. In this study we investigated the factors contributing to this variation by generating RNA-seq and Bisulfite-seq datasets of Arabidopsis thaliana plants from regenerants from two different ecotypes (Col-0 and Ws-2) and created by the ectopic activation of two different embryonic transcription factors (RKD4 and LEC2).

克隆繁殖的植株在遗传上保持完全一致，但常表现出体细胞无性系变异（somaclonal variation）。本研究通过构建两种不同生态型（Col-0与Ws-2）拟南芥（Arabidopsis thaliana）再生植株的RNA测序（RNA-seq）与亚硫酸氢盐测序（Bisulfite-seq）数据集，探究了介导此类变异的相关影响因素；所述再生植株通过异位激活两种不同的胚胎转录因子（RKD4和LEC2）获得。