Top 30 most abundantly expressed miRNAs in precursor, differentiated and hypertrophic chondrocytes from gestational day 54–56 human embryonic cartilage tissue.

Highly expressed miRNAs were identified according to their average (2-ΔCt) values. Delta (Δ) Ct value for each miRNA was calculated by subtracting the Ct value of endogenous control, RNU44, from the Ct value of the specific miRNA. Expression level average (2−ΔCt) in a region reflects the average of 2−ΔCt values across all samples in that region.

研究人员基于各miRNA的平均(2-ΔCt)值鉴定得到高表达的微小核糖核酸（miRNAs）。每个miRNA的ΔCt值，可通过将该特异性miRNA的Ct值减去内参基因RNU44的Ct值计算得到。某一区域内的(2−ΔCt)表达水平平均值，反映了该区域内所有样本的2−ΔCt值的平均水平。