DataSheet6_Real-Time Reconstruction of the Complex Field of Phase Objects Based on Off-Axis Interferometry.docx

Quantitative phase imaging (QPI) can acquire dynamic data from living cells without the need for physical contact. We presented a real-time and stable dynamic imaging system for recording complex fields of transparent samples by using Fourier transform based on off-axis interferometry. We calculated and removed the system phase without sample to obtain the real phase of the sample, so as to ensure that the system has the ability to accurately measure the phase. The temporal and spatial phase sensitivity of the system was evaluated. Benefit from the ability to record the dynamic phase and phase profile of a specimen, a standard sample (polystyrene microspheres) is investigated to demonstrate the efficiency of this imaging system and we have observed the variation of erythrocyte membrane during Red Blood Cells (RBCs) spontaneous hemolysis with different mediums. Experimental results indicate that the phase of non-anticoagulant RBC changed apparently than anticoagulant RBC and the system could be applied to real-time noninvasive and label-free identification of living cells.

定量相位成像（Quantitative Phase Imaging, QPI）可在无需物理接触的前提下获取活细胞的动态数据。本研究提出了一种基于离轴干涉术的傅里叶变换实时稳定动态成像系统，用于记录透明样品的复场信息。通过计算并去除无样品时的系统相位以获取样品的真实相位，从而保障系统可精准测量相位信息。本研究对该系统的时空相位灵敏度进行了评估。依托可记录样品动态相位及相位分布的能力，本研究以标准样品（聚苯乙烯微球）开展实验以验证该成像系统的性能，并观察了不同介质条件下红细胞（Red Blood Cells, RBCs）自发溶血过程中的红细胞膜变化。实验结果表明，非抗凝红细胞的相位变化较抗凝红细胞更为显著，该系统可用于活细胞的实时无创无标记识别。