Replication Data for: Pesticide exposure sequence and duration modify the effects of heatwaves and water level reduction on river biofilms

This dataset contains experimental data collected from controlled laboratory experiments to examine the effects of environmental stressors on biofilm microbial communities. The dataset includes measurements of both functional and structural biofilm variables. Specifically, the functional variables assessed include photosynthetic efficiency (Yeff), basal fluorescence (F0), enzyme activities (β-D-1,4-glucosidase, alkaline phosphatase, and leucine-aminopeptidase). The structural variables include chlorophyll-a concentration and algal community composition at the genus level.

The data also includes microbial gene expression, with qPCR quantification of key genes, including 16S rDNA (for bacteria and archaea) and 18S rDNA (for eukaryotes, mainly algae). Additionally, bacterial communities were characterized using high-throughput sequencing of the 16S rDNA gene. Pesticide concentrations in the water samples, as well as other water quality parameters such as nutrient concentrations, were also measured to assess the environmental conditions during the experiments.

The dataset was collected under various treatment conditions, including high temperature, pesticide exposure, and water level reduction, in both sequential and co-occurrence stressor combinations. The data are organized by experiment type (EXP1, EXP2), treatment conditions, and analysis type, providing a comprehensive resource for researchers studying microbial ecology, ecotoxicology, and environmental stress responses.

本数据集收录受控实验室实验所获实验数据，旨在探究环境胁迫因子对生物膜微生物群落的影响。数据集涵盖生物膜功能与结构两类变量的测定结果：其中功能变量包括光合效率（photosynthetic efficiency, Yeff）、基础荧光（basal fluorescence, F0）以及酶活性（涵盖β-D-1,4-葡萄糖苷酶、碱性磷酸酶与亮氨酸氨基肽酶）；结构变量则包括叶绿素a浓度以及属水平的藻类群落组成。

本数据集同时包含微生物基因表达相关数据，包括针对关键基因的定量聚合酶链反应（quantitative polymerase chain reaction, qPCR）定量结果，所涉关键基因包括用于检测细菌与古菌的16S rDNA，以及用于检测真核生物（主要为藻类）的18S rDNA。此外，研究人员通过16S rDNA基因高通量测序对细菌群落进行表征；同时还测定了水样中的农药浓度与养分浓度等其他水质参数，用以评估实验期间的环境条件。

本数据集采集自多种胁迫组合处理条件下的实验，处理方式包括高温、农药暴露与水位降低，涵盖胁迫因子先后施加与共同施加两种组合模式。数据集按照实验类型（EXP1、EXP2）、处理条件与分析类型进行组织，可为从事微生物生态学、生态毒理学及环境胁迫响应研究的科研人员提供全面的研究资源。