Locust bean gum derived galacto-manno-oligosaccharides utilization by Lactobacillus plantarum WCFS1. Locust bean gum derived galacto-manno-oligosaccharides utilization by Lactobacillus plantarum WCFS1

In order to understand LBG derived galacto-manno-oligosaccharides utilization by a probiotic bacterium, Lactobacillus plantarum WCFS1, we have grown Lactobacillus plantarum WCFS1 (in duplicates) till mid log phase (OD600nm ~0.5, 10 h) in carbon free MRS (de Man, Rogosa Sharpe ) media containing either galacto-manno-oligosaccharides, mannose, glucose or galactose (1% w/v) as the sole carbon source. Overall design: Organism : Lactobacillus plantarum, Agilent's lactobacillus Gene Expression 8x15k Array (AMADID: 067475) designed by Genotypic Technology private Limited

为解析益生菌植物乳杆菌（Lactobacillus plantarum）WCFS1对LBG衍生的半乳甘露寡糖（galacto-manno-oligosaccharides）的利用机制，我们将植物乳杆菌WCFS1设置两组生物学重复，在以1%（w/v）半乳甘露寡糖、甘露糖、葡萄糖或半乳糖为唯一碳源的无碳源MRS（de Man, Rogosa Sharpe）培养基中培养至对数生长期中期（OD600nm≈0.5，培养时长10 h）。总体实验设计：实验菌株为植物乳杆菌（Lactobacillus plantarum）；采用安捷伦（Agilent）乳杆菌基因表达8×15k芯片（阵列编号AMADID: 067475），该芯片由吉纳迪克技术私人有限公司（Genotypic Technology private Limited）定制。