Comparative transcript profiling of alloplasmic male-sterile lines revealed altered gene expression related to pollen development in rice (Oryza sativa L.). Oryza sativa

Using microarray, the anther transcript profiles of the three indica rice CMS lines revealed 622 differentially expression genes (DEGs) in each of the three CMS lines. GO and Mapman analysis indicated that these DEGs were mainly involved in lipid metabolic and cell wall organization. Comprised with the gene expression of sporophytic and gametophytic CMS lines, 303 DEGs were differentially expressed and 56 of them were down-regulated in all the CMS lines. Co-expression network analysis suggested that many genes were significantly differentially expressed in the CMS lines. These down-regulated DEGs in the CMS lines were found to be involved in tapetum or cell wall formation and their suppressed expression might be related to male sterility. The present study will give some information for the nuclear gene regulation by different cytoplasmic genotypes and provide some candidate genes for pollen development in rice. Overall design: Examine gene expression profiles of young panicles of three male sterile lines and their maitainer line.

本研究通过微阵列（microarray）技术，对3个籼稻细胞质雄性不育（Cytoplasmic Male Sterile, CMS）系的花药转录组谱进行分析，发现每个CMS系中均存在622个差异表达基因（Differentially Expressed Genes, DEGs）。基因本体（Gene Ontology, GO）富集分析与Mapman分析结果显示，这些DEGs主要参与脂质代谢与细胞壁构建过程。对比孢子体型与配子体型CMS系的基因表达谱后，共筛选得到303个差异表达基因，其中56个在所有CMS系中均呈下调表达。共表达网络分析结果表明，CMS系中存在大量显著差异表达的基因。进一步分析发现，这些在CMS系中下调的DEGs主要参与绒毡层发育与细胞壁形成过程，其表达抑制可能与雄性不育表型相关。本研究可为解析不同细胞质基因型对核基因的调控机制提供参考，并为水稻花粉发育相关研究筛选候选基因。实验设计概述：对3个雄性不育系及其对应保持系的幼穗基因表达谱进行检测。