Variations in stress sensitivity and genomic expression in diverse S. cerevisiae strains (gene expression). Saccharomyces cerevisiae

Gene expression variation was measured in 17 non-laboratory strains compared to the sequenced S288c lab strain Keywords: Gene expression comparisons in different yeast strains Overall design: Each strain was grown in at least biological triplicate to log phase in rich (YPD) medium. Extracted total RNA was compared to that collected from the diploid S288C strain, DBY8268 (ura3-52/ura3-delta, ho/ho, GAL2/GAL2)

本研究以已完成测序的实验室菌株S288c为参照，对17株非实验室酵母菌株的基因表达变异进行了检测。
关键词：不同酵母菌株间的基因表达比较
实验设计概况：所有菌株均在丰富培养基（YPD）中培养至对数生长期，且至少设置3次生物学重复；将提取的总RNA与二倍体S288C菌株DBY8268（ura3-52/ura3-delta、ho/ho、GAL2/GAL2）的总RNA进行比对。