New point mutations in the sodium channel gene and deciphering pyrethroid resistance using RNA-seq in Triatoma dimidiata from Colombia

This study focuses on determining resistance to different pyrethroids in populations of Triatoma dimidiata of Boyacá, Colombia. To determine the possible causes of resistance, we sequenced RNA of three populations: laboratory, field, and under pressure of lambda-cyhalothrin. Transcriptomic profile analyses of T. dimidiata revealed differentially regulated genes in both the field population and in the laboratory population under pressure from lambda-cyhalothrin. Gene enrichment analysis showed positive regulation of transcripts related to detoxifying enzymes and mitochondrial proteins, suggesting a significant role in resistance. To determine changes in the mRNA profiles of Triatoma dimidiata, we selected three individuals from the field population, three from the first-generation lambda-cyhalothrin pressure population, and three susceptible laboratory individuals.

本研究聚焦于检测哥伦比亚博亚卡省二带锥蝽（Triatoma dimidiata）种群对不同拟除虫菊酯类（pyrethroids）药剂的抗性水平。为明确抗性产生的潜在诱因，我们对三类种群的RNA开展了测序：实验室易感种群、野外种群以及经高效氯氟氰菊酯（lambda-cyhalothrin）胁迫的种群。针对二带锥蝽的转录组谱（transcriptomic profile）分析显示，野外种群与经高效氯氟氰菊酯胁迫的实验室种群中均存在差异调控基因。基因富集分析（gene enrichment analysis）结果表明，与解毒酶及线粒体蛋白相关的转录本呈正向调控，提示这类分子在抗性形成中发挥关键作用。为探究二带锥蝽的mRNA表达谱变化，本研究分别从野外种群、第一代高效氯氟氰菊酯胁迫种群以及易感实验室种群中各选取3头个体。