Desai SDQ0810_KNL2_N2_LTemb

modENCODE_submission_4620 This submission comes from a modENCODE project of Jason Lieb. For full list of modENCODE projects, see http://www.genome.gov/26524648 Project Goal: The focus of our analysis will be elements that specify nucleosome positioning and occupancy, control domains of gene expression, induce repression of the X chromosome, guide mitotic segregation and genome duplication, govern homolog pairing and recombination during meiosis, and organize chromosome positioning within the nucleus. 126 strategically selected targets include key histone modifications, histone variants, RNA polymerase II isoforms, dosage-compensation proteins, centromere components, homolog-pairing facilitators, recombination markers, and nuclear-envelope constituents. We will integrate information generated with existing knowledge on the biology of the targets, perform ChIP-chip analysis on mutant and RNAi extracts lacking selected target proteins, use extrachromosomal arrays to assess the ability of candidate identified sequence motifs to recruit targets in vivo, identify tissue-specific patterns of selected targets, and create integrated, quantitative models of transcription and whole-chromosome functions. For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf EXPERIMENT TYPE: CHIP-chip. BIOLOGICAL SOURCE: Strain: N2; Developmental Stage: Late Embryos; Genotype: wild type; Sex: Hermaphrodite; NUMBER OF REPLICATES: 1; EXPERIMENTAL FACTORS: Developmental Stage Late Embryos; temp (temperature) 20 degree celsius; Antibody SDQ0810 KNL-2 (target is KNL-2); Strain N2

modENCODE_submission_4620 本提交数据来自Jason Lieb主持的modENCODE项目。若需查看modENCODE项目完整列表，请访问http://www.genome.gov/26524648。
项目目标：本研究的分析重点为以下几类调控元件：介导核小体定位与占据状态的元件、调控基因表达结构域的元件、诱导X染色体沉默的元件、引导有丝分裂分离与基因组复制的元件、调控减数分裂过程中同源染色体配对与重组的元件，以及调控细胞核内染色体定位的元件。
本次筛选的126个战略性研究靶点涵盖了关键组蛋白修饰因子、组蛋白变体、RNA聚合酶II同工型、剂量补偿蛋白、着丝粒组分、同源染色体配对辅助因子、重组标记物以及核膜组分。我们将整合实验产生的数据与靶点生物学的现有知识；对缺失选定靶点蛋白的突变体及RNA干扰（RNAi）提取物开展染色质免疫沉淀芯片（ChIP-chip）分析；利用染色体外阵列（extrachromosomal arrays）技术评估候选序列基序在体内招募靶点的能力；鉴定选定靶点的组织特异性表达模式；并构建转录与全染色体功能的整合定量模型。
有关数据使用的条款与条件，请参阅http://www.genome.gov/27528022及http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf。
实验类型：染色质免疫沉淀芯片（ChIP-chip）
生物来源：菌株：N2；发育阶段：晚期胚胎；基因型：野生型；性别：雌雄同体；
重复次数：1次；
实验因素：发育阶段（晚期胚胎）；培养温度：20摄氏度；抗体：SDQ0810 KNL-2（靶点为KNL-2）；菌株：N2