Gene expression profiling of Bruguiera gymnorhiza under salt stress. Bruguiera gymnorhiza

To identify key genes in the regulation of salt tolerance in the mangrove plant Bruguiera gymnorhiza, the transcriptome profiling under salt stress was carried out. Main roots and lateral roots were collected from the mangrove plants at 0, 1, 3, 6, 12 and 24 h, 3 6 and 12 days after NaCl-treatment. Keywords: time course, stress response, root type comparison Overall design: Samples were collected from each set of three trees at 0, 1, 3, 6, 12 and 24 h, 3 6 and 12 days after NaCl-treatment, that is 27 trees were used in total. RNA was extracted from mixture of the samples from 3 trees to average genotypes of 3 trees.

为鉴定红树植物木榄（Bruguiera gymnorhiza）耐盐调控通路中的关键基因，本研究开展了盐胁迫条件下的转录组谱分析（transcriptome profiling）。研究采集了经NaCl胁迫处理后0、1、3、6、12、24小时以及3、6、12天的红树植物主根与侧根样本。
关键词：时间进程、胁迫响应、根型比较
实验设计：本研究从每组3棵植株中采集对应时间点的样本，总计使用27棵植株。将3棵植株的样本混合后提取RNA，以表征3棵植株的平均基因型特征。