IPK soil 16S rRNA sequencing. IPK soil 16S rRNA sequencing

The raw sequence reads submitted belong to 16S rRNA pyrosequencing (Roche 454) from DNA chronically contaminated soil samples treated with different concentrations of the surfactant Triton X-100 and the control without it. Triton X-100 was used as a biostimulation strategy to bioremediate the contaminated soil by solubilizing the polycyclic aromatic hydrocarbons sorbed in the soil matrix. The use of pyrosequencing—a powerful tool for analyzing complex microbial communities—in combination with the chemical determination of the sorbed and bioavailable PAH fractions enabled us to obtain strong evidence for the involvement of both physicochemical and biologic influences on the efficiency of the process.

本次提交的原始序列读段，来自对两组长期污染土壤样品DNA的16S rRNA焦磷酸测序（16S rRNA pyrosequencing，罗氏454）：一组为经不同浓度表面活性剂曲拉通X-100（Triton X-100）处理的样品，另一组为未添加该表面活性剂的对照组。曲拉通X-100被用作生物修复该污染土壤的生物刺激策略，通过溶解吸附于土壤基质中的多环芳烃（polycyclic aromatic hydrocarbons）实现修复目的。焦磷酸测序（pyrosequencing）作为解析复杂微生物群落的有力工具，与吸附态、生物可利用态多环芳烃组分的化学测定方法相结合，使我们得以获取有力证据，证明物理化学因素与生物学因素共同影响该修复过程的效率。