Peripheral blood leukocyte subsets following infection of vaccinated and naïve animals with Perth/16.

Ferrets were bled on days 0–7, 9, 11, and 13 relative to the day of viral challenge, and cells were stained and analyzed by flow cytometry as described in the text. (A) Total white blood cell counts were obtained using a Hemavet apparatus. Percentages of (B) CD3-positive cells (T cells), (C) CD8-positive cells (cytotoxic T lymphocytes), (D) CD3-positive cells (T cells) and CD8-negative cells, (E) CD79a-positive cells (B cells), and (F) CD11b-positive cells excluding CD11b-high/FSC-high cells (granulocytes) were measured. For each animal the frequencies were normalized to the ferret’s values on day 0; the Y axis represents percent of values on day 0. “Mock”, unvaccinated mock-infected animals; “Naïve”, unvaccinated animals challenged with Perth/16; “TIV”, vaccinated animals challenged with Perth/16. † indicates p<0.0001; * indicates 0.0001

本实验于病毒攻毒当日起计的第0至7日、第9、11及13日采集雪貂血液样本，并按照文中所述方法对细胞进行染色，通过流式细胞术（flow cytometry）开展分析。(A) 采用Hemavet血液分析仪获取白细胞总数。依次检测下述细胞群的占比：(B) CD3阳性细胞（T细胞）；(C) CD8阳性细胞（细胞毒性T淋巴细胞(cytotoxic T lymphocytes)）；(D) CD3阳性且CD8阴性的细胞；(E) CD79a阳性细胞（B细胞）；(F) 剔除CD11b高表达/前向散射光（FSC）高表达细胞的CD11b阳性细胞（粒细胞(granulocytes)）。每只动物的细胞频率均以其第0日的检测值作为基准进行归一化处理，纵轴代表以第0日检测值为基准的百分比。分组定义如下："Mock" 指未接种疫苗的模拟感染动物；"Naïve" 指未接种疫苗、经Perth/16毒株攻毒的动物；"TIV" 指接种疫苗、经Perth/16毒株攻毒的动物。† 代表p<0.0001；* 代表p=0.0001。