MOESM1 of Host factors determine the evolution of infection with Staphylococcus aureus to gangrenous mastitis in goats

Additional file 1. Antibody titers to the leukotoxin component LukM and α-toxin and concentration of α-toxin or LukM in milk. Antibody titers to LukM and α-toxin in serum and milk were calculated with reference to an immune goat serum arbitrarily given 1000 or 200 antibody units, respectively. Concentrations of LukM in mammary gland secretions were determined by sandwich ELISA, and of α-toxin by competitive ELISA.

附加文件1：针对白细胞毒素（leukotoxin）组分LukM与α毒素的抗体效价，以及牛奶中α毒素或LukM的浓度。血清与牛奶中针对LukM和α毒素的抗体效价，以分别人为设定为1000抗体单位与200抗体单位的免疫山羊血清为参照进行计算。乳腺分泌物中LukM的浓度采用夹心酶联免疫吸附试验（sandwich ELISA）测定，α毒素的浓度则采用竞争酶联免疫吸附试验（competitive ELISA）测定。