Environment-dependent regulation of spliceosome activity by the LSM2-8 complex in Arabidopsis. Arabidopsis thaliana

We report the role of LSM2-8 complex in the Arabidopsis tolerance to abiotic stresses. LSM2-8 controls gene expression reprogramming at the post-transcriptional level by promoting the splicing of pre-mRNA. This function is selectively achieved over selected transcripts depending on stress nature. Overall design: Transcriptomic profiling through RNA-seq of Col-0 and lsm8-1 plants exposed to low temperatures (4ºC, 24h) or high salt conditions (150 mM NaCl, 10h).

本研究阐明了LSM2-8复合物在拟南芥抵御非生物胁迫中的作用。LSM2-8可通过促进前体mRNA（pre-mRNA）的剪接，在转录后水平调控基因表达重编程，且该功能的发挥具有转录本选择性，具体取决于胁迫类型。实验设计：通过RNA测序（RNA-seq）对经低温（4℃，24小时）或高盐（150mM氯化钠，10小时）处理的Col-0与lsm8-1植株开展转录组分析。