Transcriptional profile of Candida parapsilosis CLIB214 versus UPC2 delete, both at 1% oxygen (hypoxia). Candida parapsilosis

Whole genome microarray were used to generate the transcriptional profile of C. parapsilosis UPC2 delete in hypoxia. Overall design: RNA was isolated from CLIB214 or upc2 delete grown in YPD medium in hypoxia at 30 degree Celsius for 2 h, and labeled with Cy3 or Cy5. Four independent biological replicates were compared. 2 dye swaps were perfomed so that 2 out of 4 samples isolated from CLIB214 in hypoxic culture were labeled with Cy3, and 2 were labeled with Cy5

本研究采用全基因组微阵列（Whole genome microarray）技术，获取近平滑念珠菌（Candida parapsilosis，C. parapsilosis）UPC2缺失突变株（upc2 delete）在低氧条件下的转录组谱（transcriptional profile）。实验设计概述：从经YPD培养基（YPD medium）于30℃低氧环境中培养2小时的野生型菌株CLIB214及UPC2缺失突变株中提取RNA，分别以Cy3或Cy5进行荧光标记。本实验设置4次独立生物学重复（biological replicates），并完成2次染料交换（dye swaps）实验：4份取自低氧培养的CLIB214的样本中，2份以Cy3标记，剩余2份以Cy5标记。