Gene expression during first day of collagen gel culture of rat aortic rings. Rattus norvegicus

Angiogenesis in collagen gel cultures of rat aorta begins with neovessels sprouting from the aortic explant within the first three days of culture. We used microarrays to detail the pattern of gene expression underlying initial 24 hours of growth, prior to the sprouting of visible neovessles, and identified distinct classes of up-regulated genes during this process. Overall design: Either freshly harvested aortic rings, representing day 0, or collagen gel cultures of rat aorta were grown in serum free medium and used to prepare total RNA.

大鼠主动脉胶原凝胶培养（collagen gel culture）体系中的血管生成（Angiogenesis）始于培养前三天内，新生血管（neovessels）从主动脉外植体（aortic explant）出芽萌发。本研究借助基因芯片（microarrays）详细解析了可见新生血管出芽之前的初始24小时生长过程背后的基因表达模式，并在此过程中鉴定出多类显著上调的基因。实验整体设计：将代表培养第0天的新鲜采集主动脉环，或是大鼠主动脉胶原凝胶培养体系置于无血清培养基（serum free medium）中培养，随后用于提取总RNA（total RNA）。