Hepatospora eriocheir strain:canceri Genome sequencing and assembly

In our study we constructed a six-gene concatenated phylogenetic tree for Hepatospora spp., parasites of the invasive Chinese mitten crab (Stentiford et al., 2011), a pea crab (Pinnotheres pisum) infecting microsporidium (Longshaw et al., 2012) and a novel microsporidium that infects commercially important edible crabs (Cancer pagurus), all from European waters. Our results revealed that Hepatospora isolates from the three different crustacean hosts are likely to be microvariants of a single species. Further, they support the concept that microsporidian taxonomies based upon morphology are not only unreliable, but can be deceptive, even at the level of the species. Our data, together with description of H. eriocheir as an agent of emergent disease in Asian crab aquaculture (Ding et al., 2015) reveals a preponderance for microvariants of Hepatospora eriocheir to infect the gut of a wide array of decapods crustacean hosts across wide geographic boundaries and in a range of habitats.

本研究针对肝孢子虫属（Hepatospora）物种构建了六基因串联系统发育树，所涉样本涵盖三类病原：寄生在入侵性中华绒螯蟹体内的肝孢子虫（Stentiford等，2011）、感染豆蟹（Pinnotheres pisum）的微孢子虫（microsporidium，Longshaw等，2012），以及一种可感染具有商业价值的食用蟹——普通黄道蟹（Cancer pagurus）的新型微孢子虫，所有样本均采自欧洲水域。本研究结果显示，源自三种不同甲壳类宿主的肝孢子虫分离株，大概率为同一物种的微变异株。进一步而言，本研究结果佐证了这一学术观点：基于形态学的微孢子虫分类学不仅不可靠，甚至在物种层级上也具有误导性。结合丁等（2015）将中华绒螯蟹肝孢子虫（Hepatospora eriocheir）描述为亚洲绒螯蟹养殖中新发传染病病原的研究成果，本研究数据进一步表明，中华绒螯蟹肝孢子虫的微变异株具有广泛宿主适应性：它们可感染多种十足目甲壳类动物的肠道，分布于全球不同地理区域与多样生境中。