The Impact of Long-Term Proton Pump Inhibitor Therapy on Gut Microbiota in F344 Rats. uncultured bacterium

This study was conducted to evaluate the changes in luminal microbiota composition in terminal ileum by long-term PPI treatment in aged F344 rats. Pyrosequencing of 16S ribosomal RNA (rRNA) gene has been performed by genome sequencer FLX (454 Life Sciences/Roche) and analyzed by metagenomic bioinformatics. Corresponding author: Nayoung Kim, Department of Internal Medicine, Seoul National University Bundang Hospital, Seoungnam, Gyeonggi-do, Korea; Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea (Tel., [phone]; e-mail, [e-mail]).

本研究旨在评估长期质子泵抑制剂（Proton Pump Inhibitor，PPI）处理对老年F344大鼠回肠末端管腔微生物群组成的变化影响。本研究借助基因组测序仪FLX（454 Life Sciences/Roche）对16S核糖体RNA（rRNA）基因开展焦磷酸测序，并采用宏基因组生物信息学方法完成数据分析。通讯作者：金奈英（Nayoung Kim），韩国京畿道城南市首尔大学盆唐医院内科；韩国首尔首尔大学医学院内科及肝脏研究所（电话：[phone]；电子邮箱：[e-mail]）