Effects of siERRα on gene expression in human skin fibroblast TIG113 cell.

We analyzed function of ERRα in human skin fibroblasts which silenced ERRα gene expression by microarray analysis. TIG113 was seeded in a culture dish, cultured until subconfluent, and then transfected with siRNA. After 48 h, total RNA was extracted from siRNA transfected or control TIG113 cells.

我们通过微阵列分析（microarray analysis）沉默雌激素相关受体α（ERRα）的基因表达，以此探究其在人类皮肤成纤维细胞中的功能。具体实验流程如下：将TIG113细胞接种于培养皿中，培养至亚汇合状态，随后以小干扰RNA（siRNA）进行转染。转染48小时后，分别从经siRNA转染的TIG113细胞及对照组TIG113细胞中提取总RNA。