Transcriptome profiling of pig chromosomes regions associated with water holding capacity [DuPi]. Sus scrofa

To further characterize genomic regions previously identified which are linked with meat quality traits like drip loss, PH, conductivity and etc., we employed a customary regional tiling array experiment for two pig populations. Total RNA were taken from muscle sample of M. longissimus dorsi of pigs selected from two phenotypic groups graded according to high (HI) and low (LO) drip loss. Hybridization were made in batches, 2 to 3 biological replicates for each sample. DuPi and PiF1 are code names for the two experimental populations. Overall design: Total RNA were taken from muscle sample of M. longissimus dorsi of pigs selected from two phenotypic groups graded according to high (HI) and low (LO) drip loss. Hybridization were made in batches, 2 to 3 biological replicates for each sample.

为进一步表征此前已鉴定的与滴水损失、pH值、电导率等肉品质性状相关的基因组区域，我们针对两个猪群实施了常规区域平铺芯片（regional tiling array）实验。总RNA提取自按滴水损失水平划分为高滴水损失组（HI）与低滴水损失组（LO）的两个表型分组的猪背最长肌（M. longissimus dorsi）样本。实验采用分批杂交方案，每个样本设置2至3个生物学重复。DuPi与PiF1为两个实验猪群的代号。实验整体设计：总RNA提取自按高滴水损失组（HI）与低滴水损失组（LO）分级筛选的猪背最长肌（M. longissimus dorsi）样本。实验采用分批杂交方式，每个样本设置2至3个生物学重复。