Supplementary material-Raw data for RMR determination from Is repeatability of metabolic rate influenced by social separation? A test with a teleost fish

Metabolic rates are typically thought to have important influences on fitness and more broadly be relevant to the ecology and evolution of animals. Previous studies demonstrate that metabolic rates are repeatable to a certain extent under constant conditions, but how social conditions influence the repeatability of metabolic rate remains largely unknown. In this study, we investigated the repeatability of resting metabolic rate (RMR) in the highly social crucian carp (Carassius auratus) after being socially separated for different time periods relative to control fish that were not socially separated. We found that RMR was repeatable in fish in the control group, while the repeatability of RMR disappeared quickly (even within 7 days) when fish were exposed to social separation. This study is the first to examine the role of social separation at different time periods on the repeatability of intra-individual physiological variation in fish. We highlight that the inter-individual repeatability of metabolic rate can be substantial over time but was eliminated by social separation. The findings indicate that the repeatability of metabolic rate in fish is condition dependent, and that the change in repeatability of metabolic rate should not be overlooked when considering the ecological and evolutionary effects of environmental change.

学界普遍认为，代谢率对动物的适合度具有重要影响，且在更广泛的层面上与动物的生态学及进化过程密切相关。既往研究表明，在恒定环境条件下，代谢率具有一定程度的个体间重复性，但社会环境如何影响代谢率的重复性，迄今仍不甚明确。本研究以高度社会性的鲫鱼（Carassius auratus）为实验对象，设置不同时长的社会隔离处理组，并以未经历社会隔离的个体作为对照组，探究了社会隔离后其静止代谢率（resting metabolic rate，RMR）的重复性变化。结果显示，对照组鲫鱼的静止代谢率具有显著重复性；而经社会隔离处理的鲫鱼，其静止代谢率的重复性会快速丧失，甚至在隔离7天内即可完全消失。本研究首次针对不同时长的社会隔离对鱼类个体内生理变异重复性的影响展开了系统探究。本研究指出，代谢率的个体间重复性随时间推移可维持较高水平，但社会隔离会彻底消除这一重复性。本研究结果表明，鱼类代谢率的重复性具有环境依赖性；在探讨环境变化所带来的生态学与进化效应时，代谢率重复性的变化不容忽视。