Diversity of V(D)J repertoire of germinal center (GC) and non-germinal center (NGC) B cells following W614A-3S peptide-KLH vaccination using Squalene emulsion and Alum formulation

We compared the efficacy of W614A-3S peptide formulated in either squalene emulsion (SQE) or in aluminum hydroxide (Alum) in BCR repertoire of peptide-specific B cells after 4 immunizations (at week 11 after the first intramuscular injection). The V(D)J sequencing of W614A-3S-specific BCR showed significant differences in BCR sequences and validates the dichotomy between adjuvant formulations. Four samples (one per condition) were analyzed. A pool of 25 mice per condition was use for B cell sorting.

本研究对比了分别以角鲨烯乳剂（squalene emulsion, SQE）和氢氧化铝（aluminum hydroxide, Alum）为佐剂配制的W614A-3S肽，在初次肌内注射后第11周（完成4次免疫接种后）诱导的肽特异性B细胞的B细胞受体库（BCR repertoire）的免疫效力。对W614A-3S特异性B细胞受体进行V(D)J测序的结果显示，其B细胞受体序列存在显著差异，验证了两类佐剂配方间的二分型差异。本研究共分析4份样本，每组各1份；每个实验组均采用25只小鼠的混合B细胞进行分选。