Transcriptome profiling and expression analyses of genes critical to wheat adaptation to low temperature. Triticum aestivum

Understanding the mechanism of low temperature (LT) adaptation is crucial to the development of cold-tolerant crops. To identify the genes involved in the development of LT tolerance in the crown of hexaploid wheat we examined the global changes in genes expression during cold-treatment using the Affymetrix Wheat Genome Chip. Overall design: Time-series experiment with 4 genotypes x 8 time-points X 3 biological replicates in random block design; 96 hybridizations

解析低温（low temperature, LT）适应机制对于耐寒作物的培育至关重要。为鉴定六倍体小麦分蘖节中参与LT耐受性状形成的相关基因，本研究采用Affymetrix小麦基因组芯片（Affymetrix Wheat Genome Chip）检测了冷处理过程中基因表达的全局变化。整体实验设计：该实验为时间序列实验，采用随机区组设计，设置4个基因型×8个时间点×3次生物学重复，共计完成96次杂交反应。