RNA-seq analysis of fission yeast wild type and cbf11? mutant in standard and ammonium-supplemented complex media

Transcriptome analyses of the fission yeast Schizosaccharomyces pombe cells lacking the CSL transcription factor cbf11. Wild-type cells were used as a control. Both genotypes were analyzed when grown to exponential phase in the complex YES medium, or in YES supplemented with ammonium chloride. The aim was to identify Cbf11-regulated genes, and cbf11-associated phenotypes are affected by the presence of a good nitrogen source.

针对缺失CSL转录因子（CSL transcription factor）cbf11的粟酒裂殖酵母（Schizosaccharomyces pombe）细胞开展转录组分析。以野生型细胞作为对照。将两种基因型的细胞分别在复合YES培养基或添加氯化铵的YES培养基中培养至指数生长期后进行分析。本研究旨在鉴定受Cbf11调控的基因，且发现cbf11相关表型会受优质氮源存在的影响。