Chevron_GAB Greentail prawn RNA sequences

Raw RNA sequences from 27 individual greentail prawns (Metapenaeus bennettae) collected from St Vincent Gulf in South Australia in February 2016. Prawns were placed in tanks with either clean sediment or sediment spiked with 2 mg/kg of light-weight crude oil and exposed for 24 and 90 hrs before being transferred to clean sediment tanks for a further 90 hrs. Five prawns were removed from each tank at each of the different time periods. The aprrox. 10 mg of hepatopancreas was removed and place in RNAlater before freezing at -80. RNA libraries were prepared and sequenced by the Ramaciotti Centre (Sydeny, Australia), where libraries were prepared using 1 µg RNA from 27 individuals following Illumina protocols, and sequenced on two HiSeq2500 100bp single-end lanes in accordance with the manufacturer’s protocols.

2016年2月从南澳大利亚圣文森特湾采集的27只绿尾对虾（Metapenaeus bennettae）的原始RNA序列。将对虾置于装有干净沉积物或添加了2 mg/kg轻质原油的沉积物的水箱中，分别暴露24小时和90小时后，转移至干净沉积物水箱中再放置90小时。每个时间段从每个水箱中取出5只对虾。取约10 mg肝胰腺，置于RNAlater中，随后在-80℃下冷冻。RNA文库的制备与测序由澳大利亚悉尼的拉马乔蒂中心（Ramaciotti Centre）完成：该中心遵循Illumina方案，使用27只个体的1 µg RNA制备文库，并依据制造商规程在两个HiSeq2500 100bp单端测序通道上完成测序。