Exudate - phenolphthalein pH test for evaluation of validity in seeds of Libidibia ferrea

Abstract: Seed companies are looking for promising, quick and effective alternatives to determine the physiological quality of seeds. The objective of the current work was to study the efficiency of the exudate - phenolphthalein pH test to evaluate the seeds of two lots of Libidibia ferrea (Mart. ex Tul.) L. P. Queiroz var. ferrea. The statistical design for the the exudate - phenolphthalein pH test was completely randomized with four replicates of 50 seeds in a factorial design (2 x 5), two seed lots and five soaking periods (30, 60, 90, 120, and 150 minutes), respectively, using two constant temperatures (25 and 30°C). The percentage of viability and germination of the seeds did not differ in the temperatures of 25 and 30°C and in the soaking periods by the exudate - phenolphthalein pH test. Thus, it is recommended that the test be conducted for at least 30 minutes in distilled and deionized water at the constant temperature of 25 or 30°C to evaluate the vigor of the Libidibia ferrea.

摘要：种子企业亟需可靠、高效且便捷的替代方法，以评定种子的生理品质。本研究旨在探究渗出物-酚酞pH检测法对两批Libidibia ferrea (Mart. ex Tul.) L. P. Queiroz var. ferrea种子的评估效能。该检测的实验设计采用完全随机设计，设置2×5析因处理：两个种子批次、五个浸种时长（30、60、90、120、150分钟），以及两个恒温条件（25℃与30℃），每组处理设置4个重复，每个重复含50粒种子。经渗出物-酚酞pH检测法分析，种子的活力百分比与发芽率在25℃、30℃恒温条件下，以及不同浸种时长间均无显著差异。综上，建议采用25℃或30℃恒温的蒸馏水与去离子水作为浸提介质，将检测时长设置为至少30分钟，以此评定Libidibia ferrea的种子活力。