Dissecting the dynamics of signaling events in the BMP,WNT and NODAL cascade during self-organized fate patterning in human gastruloids

To determine the in vivo cellular identity of BMP-treated human embryonic stem cells (hESCs), we performed bulk RNA sequencing data of hESCs under pluripotency and BMP-treated conditions. Our analyses show that BMP-treated hESCs resemble human trophoblast cells in vivo. mRNA profiles of four different samples, each with two biological replicates. Samples: 1) hESCs in mTeSR1 for 48h, 2) hESCs in mTeSR1+BMP4 for 48h, 3) hESCs in mTeSR1+BMP4+IWP2 for 48h, 4) hESCs in mTeSR1+BMP4+SB for 48h

为明确经骨形态发生蛋白（Bone Morphogenetic Protein, BMP）处理后人胚胎干细胞（human embryonic stem cells, hESCs）的体内细胞身份，我们对处于多能性状态及经BMP处理条件下的hESCs进行了批量RNA测序（bulk RNA sequencing）。分析结果显示，经BMP处理的hESCs与体内人滋养层细胞具有相似的细胞特征。本数据集包含4组独立样本，每组均设置2次生物学重复，具体样本信息如下：1）于mTeSR1培养基中培养48小时的hESCs；2）于含mTeSR1+BMP4的培养基中培养48小时的hESCs；3）于含mTeSR1+BMP4+IWP2的培养基中培养48小时的hESCs；4）于含mTeSR1+BMP4+SB的培养基中培养48小时的hESCs。