Transcriptomic response of MDA-MB-231 breast cancer cell line in treatment with graphene oxide. Homo sapiens

Graphene-based materials have recently emerged as promising materials in cancer therapy.To find the molecular mechanisms and key regulatory molecules related to graphene-induced cytotoxicity used high throughput sequencing.In this study reduced graphene oxide-(L-lysine) was selected for a high-throughput investigation. Using mRNA-sequencing, ~6 Giga base clean data (20 Million Reads, 150 base pair) per individual sample and totally 125.3 million reads of transcriptome sequence were generated. Gene expression study highlighted the behavior of the graphene oxide-derivate through reducing the expression of several genes involved in the cell migration, extracellular adhesion process, and cell connection.

近年来，基于石墨烯的材料已成为癌症治疗领域极具应用前景的候选材料。为探明石墨烯诱导细胞毒性相关的分子机制及关键调控分子，本研究采用高通量测序（high throughput sequencing）技术开展相关研究。本研究选取还原氧化石墨烯-(L-赖氨酸)（reduced graphene oxide-(L-lysine)）作为研究对象进行高通量分析。通过mRNA测序（mRNA-sequencing），每个独立样本可获得约6吉碱基（Giga base）的清洁序列数据（含2000万条读段（Reads），读长150碱基对），本次转录组测序共生成1.253亿条读段。基因表达分析结果显示，该氧化石墨烯衍生物可通过下调多个参与细胞迁移、细胞外黏附过程及细胞连接的基因的表达，展现出相应的生物学调控效应。