Use of heteropolymeric monoclonal antibodies to attach antigens to the C3b receptor of human erythrocytes: a potential therapeutic treatment.

We have prepared bispecific, cross-linked monoclonal antibodies (heteropolymers) with specificity for both targeted antigens and the human erythrocyte (RBC) complement receptor. These heteropolymers facilitate binding of target antigens (human IgG and dinitrophenylated bovine gamma globulin) to human RBCs under conditions that either allow or preclude complement activation. Quantitative analyses of this binding agree well with the number of complement receptors per RBC. In vitro "whole-blood" model experiments indicate heteropolymer-facilitated binding of antigens to RBCs is rapid and stable at 37 degrees C. It may be possible to extend these prototype experiments to the in vivo situation and use heteropolymer-attached RBCs for the safe and rapid binding, neutralization, and removal from the circulation of pathogenic antigens associated with infectious disease.

本研究制备了可同时结合靶抗原与人红细胞（RBC）补体受体（complement receptor）的双特异性交联单克隆抗体（异多聚体，heteropolymers）。该类异多聚体可在允许或抑制补体激活的条件下，介导靶抗原（人免疫球蛋白G（human IgG）及二硝基苯基化牛γ球蛋白）与人红细胞的结合。对该结合过程的定量分析结果，与每个红细胞表面补体受体的数量高度吻合。体外"全血"模型实验结果显示，在37℃环境下，异多聚体介导的抗原与红细胞结合过程快速且稳定。有望将此类原型实验拓展至体内场景，并利用结合有异多聚体的红细胞，安全、快速地完成结合、中和以及清除循环中与传染病相关的致病性抗原。