Intermittent childhood hyperlipidaemia alters resident arterial macrophages and accelerates atherosclerosis. Intermittent childhood hyperlipidaemia alters resident arterial macrophages and accelerates atherosclerosis

Peritoneal macrophages were obtained by intraperitoneal injection of cold PBS from Ldlr−/− mice reconstituted with Lyz2Cre+/− Nrp1flox/flox or Lyz2Cre+/− Nrp1+/+ bone marrow.

从经溶菌酶2（Lyz2）Cre+/− 神经纤毛蛋白1（Nrp1）条件性敲除纯合（Nrp1flox/flox）或Lyz2Cre+/− Nrp1+/+骨髓重建的低密度脂蛋白受体基因敲除（Ldlr−/−）小鼠体内，通过腹腔注射冰冷磷酸盐缓冲液（PBS），可获取腹腔巨噬细胞（peritoneal macrophages）。