Microarray analysis of gene expression in liver, adipose tissue and skeletal muscle in response to chronic dietary administration of NDGA to high-fructose fed dyslipidemic rats. Rattus norvegicus

A high-fructose diet (HFrD) fed rat model of hypertriglyceridemia, dyslipidemia, insulin resistance and hepatic steatosis was employed to investigate the global transcriptional changes in the lipid metabolizing pathways in three insulin sensitive tissues:, liver, skeletal muscle and adipose tissue in response to chronic dietary administration of NDGA. Overall design: Sprague-Dawley male rats (SD) were fed a chow (control) diet, high-fructose diet (HFrD) or HFrD supplemented with NDGA (2.4 g/kg diet) for eight weeks. Dietary administration of NDGA decreased plasma levels of TG, glucose, and insulin, and attenuated hepatic TG accumulation. DNA microarray expression profiling was performed on RNA isolated from liver, adipose and muscle.

本研究采用高果糖饮食（high-fructose diet, HFrD）构建的高甘油三酯血症、血脂异常、胰岛素抵抗及肝脂肪变大鼠模型，以探究长期膳食给予去甲二氢愈创木脂酸（NDGA）后，肝脏、骨骼肌与脂肪组织这三种胰岛素敏感组织内脂质代谢通路的全局转录变化。实验设计：将雄性Sprague-Dawley（SD）大鼠分为三组，分别饲喂普通维持饲料（对照组）、高果糖饮食（HFrD），以及添加2.4 g/kg NDGA的高果糖饮食，干预周期为8周。结果显示，NDGA给药可降低血浆甘油三酯（TG）、葡萄糖及胰岛素水平，并减轻肝脏TG蓄积。研究人员从肝脏、脂肪组织与骨骼肌中提取总RNA，开展DNA微阵列表达谱分析。