Comparison of transcriptomic profiles of Pseudomonas protegens H78 and its rpoN-deleted mutant (H78?rpoN)

Transcriptomic profiles of Pseudomonas protegens H78 and its rpoN-deleted mutant (H78?rpoN), which were grown to the late exponential phase (OD600 = 4.0 to 5.0) in the KMB media at 28 °C, were assessed by deep sequencing (RNA-seq) on BGISEQ-500 (illumina). Overall design: Two bacterial strains, Pseudomonas protegens H78 wild type strain and its rpoN-deleted mutant strain (H78?rpoN), each was replicated three times.

本数据集收录产假单胞菌（Pseudomonas protegens）H78及其rpoN基因缺失突变株（rpoN-deleted mutant）H78ΔrpoN的转录组谱（Transcriptomic profiles）。上述菌株于28℃下在KMB培养基（KMB media）中培养至指数生长后期（此时OD600值为4.0至5.0），随后通过BGISEQ-500（因美纳Illumina）测序平台开展深度测序（RNA测序，RNA-seq）分析。实验设计概述：共设置两类细菌菌株，即产假单胞菌（Pseudomonas protegens）H78野生型菌株及其rpoN基因缺失突变株（rpoN-deleted mutant）H78ΔrpoN，每类菌株均设置3次生物学重复（biological replicates）。